Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Isolating Malignant and Non-Malignant B Cells…
JoVE Journal
Medicine

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Medicine
Isolating Malignant and Non-Malignant B Cells from lck
 

Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish

Article DOI: 10.3791/59191-v • 08:32 min • February 22nd, 2019
February 22nd, 2019 •
1, *1, *1, 1, 1
1Section of Pediatric Hematology-Oncology, Department of Pediatrics, University of Oklahoma Health Sciences Center
* These authors contributed equally

Chapitres

résumé
Automatic Translation

Transgenic lck:eGFP zebrafish express GFP highly in T lymphocytes, and have been used to study T cell development and acute lymphoblastic leukemia. This line can be used to study B cells, which express lck at lower levels. This protocol describes purification of malignant and non-malignant B cells from lck:eGFP zebrafish.

Tags

Isolate Malignant B Cells Non-malignant B Cells Lck:eGFP Zebrafish Study T Cells Technique B-ALL T-ALL Cells Fish With Cancer B Cell Development Adaptive Immune System Malignancies Pediatric Cancer Dissections Thymus Samples Marrow Samples Visual Demonstration Fluorous Anesthetizing Tricaine Epifluorescence Microscope GFP Filter Fluorescent Thymi Dorsal Medial Aspect Of The Branchial Cavity Petri Dish Fluorescent Microscope GFP Positive Thymi Brightfield Microscopy Settings Kidney Marrow Spleen Sorting Medium
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter