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Biochemistry

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JoVE Journal Biochemistry
A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput
 

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

Article DOI: 10.3791/59333-v • 12:22 min • September 22nd, 2019
September 22nd, 2019 •
1, 1, 1, 1
1National Institute of Neurological Disorders and Stroke (NINDS), Neurotherapeutic Development Unit (NTDU), National Institutes of Health (NIH)

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It is often necessary to assess the potential cytotoxicity of a set of compounds on cultured cells. Here, we describe a strategy to reliably screen for toxic compounds in a 96-well format.

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Cell Growth Cell Survival Compound Screening Cytotoxic Compounds Assay Cellular Function Dose Response Curve Primary Screen Cell Culture T75 Flask NSC Medium Incubator
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