Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Sequential Immunofluorescence and…
JoVE Journal
Biology

Ce contenu est en libre accès.

JoVE Journal Biology
Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
 

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Article DOI: 10.3791/59344-v • 09:20 min • May 14th, 2019
May 14th, 2019 •
,
1Department of Population Health and Pathobiology, NC State University College of Veterinary Medicine

Chapitres

résumé
Automatic Translation

This protocol demonstrates sequential immunofluorescence and immunohistochemistry on cryosections from early-stage zebrafish embryos enabling precise colocalization analyses in specific cell populations.

Tags

Keywords: Immunofluorescence Immunohistochemistry Cryosections Zebrafish Embryos Co-localization Antibody Compatibility Cryosectioning Tissue Morphology Co-expression Embryo Orientation Cryostat Charged Glass Slides PBS Wash Humid Chamber Barrier Pen
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter