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Bioengineering

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JoVE Journal Bioengineering
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
 

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Article DOI: 10.3791/59350-v • 10:18 min • May 28th, 2019
May 28th, 2019 •
1,2, 1, 3,4, 1, 1
1DSM Biotechnology Center, 2Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, 3BrisSynBio, University of Bristol, Life Sciences Building, 4School of Biological Sciences, University of Bristol, Life Sciences Building

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The CRISPR/Cas12a system in combination with a single crRNA array enables efficient multiplex editing of the S. cerevisiae genome at multiple loci simultaneously. This is demonstrated by constructing carotenoid producing yeast strains which are subsequently used to create yeast pixel art.

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CRISPR/Cas12a Multiplex Genome Editing Saccharomyces Cerevisiae Yeast Pixel Art Cloning-free Plasmid Assembly CrRNA Array RNA Polymerase Cas12a Double-strand Breaks In Vivo Recombination Genomic DNA Klaudia Ciurkot Jeffery Van Wijk Brenda Vonk PCR Amplification Thermocycler Electrophoresis Agarose Gel
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