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JoVE Journal Neuroscience
Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase
 

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

Article DOI: 10.3791/59446-v • 09:53 min • April 23rd, 2019
April 23rd, 2019 •
1, 1,2, 1,3, 1,4, 1,3,5,6
1Department of Molecular Genetics and Microbiology, Duke University Medical Center, 2Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 3Department of Neurobiology, Duke University Medical Center, 4Department of Mechanical Systems, Engineering, Tokyo University of Agriculture and Technology, 5Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 6Duke Institute for Brain Sciences, Duke University

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Physiologically, odorant receptors are activated by odorant molecules inhaled in the vapor phase. However, most in vitro systems utilize liquid phase odorant stimulation. Here, we present a method that allows real-time in vitro monitoring of odorant receptor activation upon odorant stimulation in vapor phase.

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Keywords: Odorant Receptor In Vitro Monitoring Vapor Phase Biosensor Odor Perception Cell Culture Real-time M10 PSF Trypsin-EDTA 96-well Plate
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