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Developmental Biology

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JoVE Journal Developmental Biology
Microscopia isotropica leggera e analisi di lignaggio cellulare automatizzate al catalogo Caenorhabditis elegans embriogenesi con risoluzione subcellulare
 
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Microscopia isotropica leggera e analisi di lignaggio cellulare automatizzate al catalogo Caenorhabditis elegans embriogenesi con risoluzione subcellulare

Article DOI: 10.3791/59533-v • 08:16 min • June 6th, 2019
June 6th, 2019 •
1,5, 1,5, 1,5, 1,5, 1,5, 4,5, 4,5, 4,5, 2,5, 2,5, 4,5, 3,5, 1,5,6
1Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine, 2Developmental Biology Program, Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling, University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico

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Qui, presentiamo un approccio combinatorio utilizzando microscopia ad alta risoluzione, strumenti computazionali, e l'etichettatura a cella singola in vivente embrioni C. elegans per comprendere le singole dinamiche delle cellule durante il neurosviluppo.

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Biologia dello sviluppo problema 148 diSPIM CytoSHOW deconvoluzione SpimFusion StarryNite visualizzazione lignaggio neurosviluppo C. elegans embrioni
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