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Biochemistry

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JoVE Journal Biochemistry
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
 

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)

Article DOI: 10.3791/59615-v • 10:00 min • June 20th, 2019
June 20th, 2019 •
1, 2, 3, 2
1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.

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résumé
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This protocol describes the combination of size exclusion chromatography with multi-angle light scattering (SEC-MALS) for absolute characterization of proteins and complexes in solution. SEC-MALS determines the molecular weight and size of pure proteins, native oligomers, heterocomplexes and modified proteins such as glycoproteins.

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Keywords: SEC-MALS Size-exclusion Chromatography Multi-angle Light Scattering Molecular Weight Macromolecules Oligomers Complexes Glycoproteins Membrane Proteins Absolute Method Peptides Polymers Proteins Nucleocapsids Polysaccharides Virus-like Particles Viruses Phosphate-buffered Saline Flow Rate DRI Flow Cell Purge Mode System Cleanliness Refractive Index
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