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Bioengineering

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JoVE Journal Bioengineering
In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
 

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Article DOI: 10.3791/59647-v • 05:45 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1
1School of Brain and Behavioral Science, Center for Advanced Pain Studies, University of Texas at Dallas

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Morphological changes occur in immune responsive fibroblast cells following activation and promote alterations in cellular recruitment. Utilizing 2-photon imaging in conjunction with a genetically engineered Fibroblast-specific protein 1 (FSP1)-cre; tdTomato floxed-stop-floxed (TB/TB) mouse line and green fluorescently tagged lipopolysaccharide-FITC, we can illustrate highly specific uptake of lipopolysaccharide in dermal fibroblasts and morphological changes in vivo.

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In Vivo Two-Color 2-Photon Imaging Genetically-Tagged Reporter Cells Skin Fluorescently Tagged Cells Inflammatory Peripheral Stimulus Live Animal Real Time 2-Photon Imaging Tissue Visualization Microenvironment Integrity Biological System Stable Surface Objective Placement Multi-photon System 25X Subjective Stereotaxic Apparatus Anesthesia Delivery Machine Matte Black Paper Resonance Scanner Excitation Lasers Green And Red Fluorescent Protein Signals Dichroic Mirror
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