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Biochemistry

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JoVE Journal Biochemistry
Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
 

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription

Article DOI: 10.3791/59687-v • 07:18 min • July 26th, 2019
July 26th, 2019 •
1,2, 3, 1,2
1Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers University, 2Graduate Program in Physiology and Integrative Biology, School of Graduate Studies, Rutgers University, 3Department of Plant Biology, School of Environmental and Biological, Rutgers University

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Interactions of transcription factors (TFs) with the RNA polymerase are usually studied using pulldown assays. We apply a Biolayer Interferometry (BLI) technology to characterize the interaction of GrgA with the chlamydial RNA polymerase. Compared to pulldown assays, BLI detects real-time association and dissociation, offers higher sensitivity, and is highly quantitative.

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Keywords: Biolayer Interferometry BLI Protein-protein Interactions Transcription Kinetic Parameters Biosensor Nickel NTA Real-time Association And Dissociation Quantitative Analysis Por Dialysis
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