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JoVE Journal Cancer Research
Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells
 

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Article DOI: 10.3791/59696-v • 10:03 min • July 5th, 2019
July 5th, 2019 •
*1, *1, *1, *2, *1, *2, *2, 1,2,3,4
1Department of Biomedical Engineering, University of Michigan, 2Department of Materials Science and Engineering, University of Michigan, 3Rogel Cancer Center, School of Medicine, University of Michigan, 4Macromolecular Science and Engineering, University of Michigan
* These authors contributed equally

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résumé
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This protocol describes generation of patient-derived spheroids, and downstream analysis including quantification of proliferation, cytotoxicity testing, flow cytometry, immunofluorescence staining and confocal imaging, in order to assess drug candidates’ potential as anti-neoplastic therapeutics. This protocol supports precision medicine with identification of specific drugs for each patient and stage of disease.

Tags

Physiologic Patient-derived 3D Spheroids Anti-neoplastic Drug Screening Cancer Stem Cells 3D Cell Culture Downstream Analysis Heterogeneous Patient Samples Specific Drug Responses Spheroid Formation Preservation Of Primary Samples High Throughput Screening Hanging Drop Model Physiological Environment 3D Drug Diffusion Tumor Stage Responses Targeted Therapies Patient-specific Treatments Ovarian Cancer Heterogeneity Chemo Resistance Drug-resistant Cell Populations Pipette Precise Volumes Consistency Between Spheroids Fragile Spheroids And Droplets Proper Handling Hanging Drop Plate Analysis
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