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Bioengineering

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JoVE Journal Bioengineering
Optisk billeddannelse af isolerede murine ventrikulære Myocytter
 
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Optisk billeddannelse af isolerede murine ventrikulære Myocytter

Article DOI: 10.3791/60196-v • 11:32 min • January 17th, 2020
January 17th, 2020 •
1,2, 3, 3, 3, 3, 1, 3, 3
1Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China, 2Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, 3Pediatric Cardiac and Thoracic Surgery, University Hospitals Cleveland Medical Center

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Vi præsenterer metoden til isolering af murine myocytter og hvordan man får spænding eller calcium spor samtidig med sarcomere afkortning af spor ved hjælp af fluorescens fotometri med samtidige digitale celle geometri målinger.

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Retraktion myocyte enzymatisk isolation optisk aktionspotentiale calcium excitation-sammentrækning kobling action potentiel varighed
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