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Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods
 

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods

Article DOI: 10.3791/60335-v 10:24 min November 19th, 2019
November 19th, 2019

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résumé

We present a flow cytometry method to identify simultaneously different cell types retrieved from mouse brain or spinal cord. This method could be exploited to isolate or characterize pure cell populations in neurodegenerative diseases or to quantify the extent of cell targeting upon in vivo administration of viral vectors or nanoparticles.

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Keywords: Flow Cytometry Cell Characterization Mouse Brain Spinal Cord Tissue Homogenization Cell Isolation Neural Tissue Dissociation Gene Delivery Drug Delivery Neurodegenerative Disorders
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