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Developmental Biology

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JoVE Journal Developmental Biology
In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads
 

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

Article DOI: 10.3791/60422-v • 07:52 min • November 26th, 2019
November 26th, 2019 •
1,2, 1,2, 1,2
1Department of Zoology, The University of British Columbia, 2Life Sciences Institute, The University of British Columbia

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Tissue complexities of multicellular systems confound the identification of causal relationship between extracellular cues and individual cellular behaviors. Here, we present a method to study the direct link between contact-dependent cues and division axes using C. elegans embryo blastomeres and adhesive polystyrene beads.

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In Vitro Reconstitution Spatial Cell Contact Patterns Caenorhabditis Elegans Embryo Blastomeres Adhesive Polystyrene Beads Cellular Conduct Chemically Functional Polystyrene Beads Cellular Response C-Elegance Mouse Embryos Chlorite Treatment Blastomere Isolation Methods Carboxylate Modified Polystyrene Beads MES Buffer Bench-top Centrifuge EDAC Activation
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