JoVE Journal
Biochemistry
Biochemistry
Ce contenu est en libre accès.
Chapitres
résumé
A method for transcriptome profiling of cereals is presented. The microarray-based gene expression profiling starts with the isolation of high-quality total RNA from cereal grains and continues with the generation of cDNA. After cRNA labelling and microarray hybridization, recommendations are given for signal detection and quality control.