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Immunology and Infection

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JoVE Journal Immunology and Infection
TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
 

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

Article DOI: 10.3791/60728-v • 07:02 min • May 17th, 2020
May 17th, 2020 •
*1, *2,3, 1, 1, 2, 2,3
1State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, 2Department of Plant Biology, College of Biological Sciences, University of California, Davis, 3Genome Center, College of Biological Sciences, University of California, Davis
* These authors contributed equally

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Described here is a proximity labeling method for identification of interaction partners of the TIR domain of the NLR immune receptor in Nicotiana benthamiana leaf tissue. Also provided is a detailed protocol for the identification of interactions between other proteins of interest using this technique in Nicotiana and other plant species.

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Keywords: TurboID Proximity Labeling Protein-protein Interactions NLR Immune Receptors Nicotiana Benthamiana Agroinfiltration Biotin RIPA Lysis Buffer Desalting Column
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