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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifiering av neutrofila extracellulära fällor i paraffinin embedded felintens arteriell trombi med immunofluorescensmikroskopi
 
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Identifiering av neutrofila extracellulära fällor i paraffinin embedded felintens arteriell trombi med immunofluorescensmikroskopi

Article DOI: 10.3791/60834-v • 08:48 min • March 29th, 2020
March 29th, 2020 •
1, 2, 3, 2
1Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, 2Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 3Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis

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Vi beskriver en metod för att identifiera neutrophil extracellulära fällor (ELT) i formaldehyde-fasta och paraffin-inbäddade feline cardiogenic kranskärlens trombi med värme-inducerad antigen hämtning och en dubbel immunmärkning protokoll.

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Immunologi och infektion Nummer 157 immunofluorescensmikroskopi citrullinerad histon H3 neutrofil elastas arteriell trombos felint hypertrofisk kardiomyopati
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