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Biochemistry

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JoVE Journal Biochemistry
Umålrettet flytende kromatografi-massespektrometribasert metabolomikkanalyse av hvetekorn
 
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Umålrettet flytende kromatografi-massespektrometribasert metabolomikkanalyse av hvetekorn

Article DOI: 10.3791/60851-v • 07:10 min • March 13th, 2020
March 13th, 2020 •
1,2,3, 1,4, 5,6, 1
1Research and Innovation, Murdoch University, 2Centre for Digital Agriculture, Curtin University, 3Centre for Integrative Metabolomics and Computational Biology, School of Science, Edith Cowan University, 4Centre for Computational and System Medicine, Health Futures Institute, Murdoch University, 5Department of Primary Industries and Regional Development, 6State Agricultural Biotechnology Centre, Murdoch University

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En metode for umålrettet analyse av hvetekornmetabolitter og lipider presenteres. Protokollen inkluderer en acetonitrile metabolitt ekstraksjonsmetode og reversert fase flytende kromatografi-massespektrometri metodikk, med oppkjøp i positive og negative elektrospray ioniseringmoduser.

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Biokjemi hvete feltdyrket hvete hvete variasjon hvetekorn landbruk metabolomikk umålrettede metabolomikk flytende kromatografi massespektrometri
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