Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Laser Capture Microdissection of Glioma…
JoVE Journal
Neuroscience

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Neuroscience
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
 

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Article DOI: 10.3791/60939-v • 09:09 min • April 12th, 2020
April 12th, 2020 •
1,2,4, 1,2,4, 1,3, 1,2,4, 3, 1,2,4, 1,2,4
1Dept. of Neurosurgery, University of Michigan Medical School, 2Dept. of Cell and Developmental Biology, University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science, University of Michigan Medical School, 4Rogel Cancer Center, University of Michigan Medical School

Chapitres

résumé
Automatic Translation

Laser microdissection (LMD) is a sensitive and highly reproducible technique that can be used to uncover pathways that mediate glioma heterogeneity and invasion. Here, we describe an optimized protocol to isolate discrete areas from glioma tissue using laser LMD followed by transcriptomic analysis.

Tags

Keywords: Laser Microdissection RNA Sequencing Intratumor Heterogeneity Mesenchymal Cells Tumor Invasion Tumor Morphology Molecular Profiles Tumor Progression Therapeutic Targets Bioluminescence Perfusion Cryopreservation Frozen Brain Tissue Spatial Characterization Molecular Characterization
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter