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Biochemistry

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JoVE Journal Biochemistry
Karakterisering van Amyloïde Structuren in Aging C. Elegans met behulp van Fluorescentie Lifetime Imaging
 
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Karakterisering van Amyloïde Structuren in Aging C. Elegans met behulp van Fluorescentie Lifetime Imaging

Article DOI: 10.3791/61004-v • 09:31 min • March 27th, 2020
March 27th, 2020 •
*1,2, *1, *1, 3, 1,4
1Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin, 2NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, 3Molecular Neuroscience Group, Department of Chemical Engineering and Biotechnology, University of Cambridge, 4Cell Biology, University of Bremen
* These authors contributed equally

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Fluorescentie levenslange beeldvorming monitoren, kwantificeert en onderscheidt de aggregatie tendensen van eiwitten in het leven, veroudering, en benadrukt C. elegans ziekte modellen.

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Biochemie C. elegans aggregatie veroudering proteostase netwerk siRNA knockdown fluorescentie lifetime imaging microscopie (FLIM) time-correlated single photon counting (TCSPC) lifetime (tau)
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