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Measuring RAN Peptide Toxicity in C. elegans
 

Measuring RAN Peptide Toxicity in C. elegans

Article DOI: 10.3791/61024-v • 10:49 min • April 30th, 2020
April 30th, 2020 •
1, 2, 2, 1,2
1Graduate Program in Cell Biology and Molecular Physiology, University of Pittsburgh, 2Department of Pediatrics, University of Pittsburgh School of Medicine

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Repeat-associated non-ATG-dependent translational products are emerging pathogenic features of several repeat expansion-based diseases. The goal of the protocol described is to evaluate toxicity caused by these peptides using behavioral and cellular assays in the model system C. elegans.

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RAN Peptides Repeat Expansion Neurodegenerative Disorders Huntington's Disease Amyotrophic Lateral Sclerosis Frontotemporal Dementia C. Elegans Model System Quantifying RAN Peptide Toxicity Standardized Approach Techniques Simple To Learn Inexpensive Quick Performance Reproducible Features Movement And Reproduction Consistent RAN Peptide Toxicity And Expression Drug Temperature Control Timing Of Temperature Shifts Assay Plate Preparation Phenotypes Classification Age-dependent Process Assay Visual Demonstration Nematode Growth Medium IPTG Concentration Carbenicillin Concentration RNAi Feeding Clones LB Carbenicillin Agar Bacteria Growth Temperature
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