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A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT
 

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Article DOI: 10.3791/61081-v • 07:10 min • June 12th, 2020
June 12th, 2020 •
1, 1, 2
1Department of Biology, The University of Akron, 2Department of Geosciences, The University of Akron

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We employed a geological (coring) sampling protocol to procure cortical bone specimens of uniform size for SRµCT experiments from the anterior aspect of human femora. This method is minimally destructive, efficient, results in cylindrical specimens that minimize imaging artifacts from irregular sample shapes and improves microarchitectural visualization and analysis.

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Keywords: Bone Specimen Cortical Bone Synchrotron Micro-CT Sample Procurement Sectioning Coring Image Processing High-throughput Thermal Epoxy Resin Microscope Slide Slow Speed Sectioning Saw Cross-section Rock Coring Fossil Coring Hard Material Coring Earth's Crust Upper Mantle Rheology
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