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Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy
 

Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy

Article DOI: 10.3791/61105-v • 08:56 min • May 8th, 2020
May 8th, 2020 •
1, 1, 1
1Department of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw

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Presented here is a protocol that describes monitoring of the complete life cycle of predatory bacterium Bdellovibrio bacteriovorus using time-lapse fluorescence microscopy in combination with an agarose pad and cell-imaging dishes.

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Keywords: Live-cell Imaging Bdellovibrio Bacteriovorus Time-lapse Fluorescence Microscopy Bacterial Predator Life Cycle Prey Cells Agarose Pad Calcium HEPES Buffer Optical Density Centrifugation Fluorescence Microscopy Prey Lysis Multi-attack Phase
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