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Genetics

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JoVE Journal Genetics
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
 

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Article DOI: 10.3791/61231-v • 08:50 min • May 14th, 2020
May 14th, 2020 •
*1, *2, 3, 1
1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School, University of Padua, 3Biologie de l'Ecole Normale Supérieure (IBENS), Paris, France
* These authors contributed equally

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The methylated RNA immunoprecipitation assay is an antibody-based method used to enrich for methylated RNA fragments. Coupled with deep sequencing, it leads to the identification of transcripts carrying the m5C modification.

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Methylated RNA Immunoprecipitation Assay MeRIP RNA Modification Arabidopsis RNA Extraction Guanidine Thiocyanate Acid Phenol 1-bromo-3-chloropropane Isopropanol Sodium Acetate Precipitation Centrifugation Ethanol Wash
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