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Bioengineering

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JoVE Journal Bioengineering
An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion
 

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

Article DOI: 10.3791/61261-v • 08:29 min • March 31st, 2022
March 31st, 2022 •
1,2, 1,3, 1, 1, 1,3,4,5, 1,2, 1,2,3,4,6
1Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation, Queensland University of Technology, 2School of Mechanical, Medical and Process Engineering, Science and Engineering Faculty, Queensland University of Technology, 3School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, 4Australian Prostate Cancer Research Centre, Institute of Health and Biomedical Innovation, Queensland University of Technology, 5Translational Research Institute, Queensland University of Technology, 6ARC ITTC in Additive Biomanufacturing, Institute of Health and Biomedical Innovation, Queensland University of Technology

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résumé
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This protocol serves as a comprehensive tutorial for standardized and reproducible mixing of viscous materials with a novel open source automation technology. Detailed instructions are provided on the operation of a newly developed open source workstation, the usage of an open source protocol designer, and the validation and verification to identify reproducible mixtures.

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Keywords: Open Source Biomedical Research Reproducibility Automation Standardization Hydrogel 3D Cell Culture Tissue Engineering Manufacturing API Pipetting Biofabrication Protocol Design GelMA Alginate Photoinitiator Photo Crosslinking 96-well Plate Double Network Hydrogels Advanced Mixing Protocol
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