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Immunology and Infection

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JoVE Journal Immunology and Infection
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
 

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Article DOI: 10.3791/61348-v • 09:58 min • June 17th, 2020
June 17th, 2020 •
1, *2, *1
1Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

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résumé
Automatic Translation

This protocol provides a method to digest whole eyes into a single cell suspension for the purpose of multi-parameter flow cytometric analysis in order to identify specific ocular mononuclear phagocytic populations, including monocytes, microglia, macrophages, and dendritic cells.

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Digestion Mouse Eyes Flow Cytometric Analysis Mononuclear Phagocytes Macrophages Monocytes Microglia Dendritic Cells Quantitative Analysis Fluorophore Intensity Cell Surface Marker Expression Macrophage Heterogeneity Laser-induced Choroidal Neovascularization Model Diabetic Retinopathy Experimental Autoimmune Uveitis Lupus Manifestations Cytometer Configuration Fluorescently Conjugated Antibodies Protocol Optimization Digestion Buffer Preparation Dissection Microscope
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