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Immunology and Infection

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JoVE Journal Immunology and Infection
Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
 

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Article DOI: 10.3791/62985-v • 09:57 min • November 16th, 2021
November 16th, 2021 •
1,2, 1,2,3, 1,2, 1,2, 1,2
1Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 3Harvard College

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résumé
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In this study, we present an effective and reproducible protocol to isolate the immune populations of the murine respiratory system. We also provide a method for the identification of all innate and adaptive immune cells that reside in the lungs of healthy mice, using a 9-color-based flow cytometry panel.

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Lung Immune Cells Myeloid Cells Non-myeloid Cells Flow Cytometry Gating Strategy Lung Tissue Digestion Lung Immune Landscape
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