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JoVE Journal Bioengineering
Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts
 

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Article DOI: 10.3791/61404-v • 09:55 min • May 16th, 2020
May 16th, 2020 •
*1, *1, 1, 1, 1,2
1Physiopathogénèse et traitement des maladies du foie, Université Paris-Saclay, Inserm U1193, 2ESPCI Paris, Université PSL
* These authors contributed equally

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résumé
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Three-dimensional (3D) cellular systems are relevant models for investigating organogenesis. A hydrogel-based method for biliary cysts production and their characterization is proposed. This protocol unravels the barriers of 3D characterization, with a straightforward and reliable method to assess cyst formation efficiency, sizes, and to test their functionality.

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Keywords: Biliary Epithelial Cysts Hydrogel Normal Rat Cholangiocytes Cell Culture Cyst Formation Quantitative Assessment Tubular Structures Bioengineering Therapeutic Targets Disease Mechanisms
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