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Immunology and Infection

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JoVE Journal Immunology and Infection
Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
 

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Article DOI: 10.3791/61414-v • 09:02 min • June 5th, 2020
June 5th, 2020 •
*1,2,3, *1,2,3,4, 1,2,3, 1,2,3,5, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Stem Cell Research Center, Shantou University Medical College, 2The Center for Reproductive Medicine, Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications
* These authors contributed equally

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résumé
Automatic Translation

This manuscript describes a detailed protocol for Hepatitis B virus (HBV) infection in novel engineered 293T cells (293T-NE-3NRs, expressing human NTCP, HNF4α, RXRα and PPARα) and traditional hepatic cells (HepG2-NE, expressing human NTCP).

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Keywords: Hepatitis B Virus HBV Non-hepatic Cells 293T-NE-3NR In Vitro Model HepG2.2.15 Cell Culture Viral Replication Extrahepatic Syndromes Cell Line Engineering Biosafety Viral Supernatant Virus Concentration Real-time PCR
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