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Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
 

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Article DOI: 10.3791/61461-v • 06:46 min • June 24th, 2020
June 24th, 2020 •
1, 1, 1, 1, 1, 1
1Maria Sklodowska-Curie National Research Institute of Oncology

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The article describes quantification of 1) the size and number of focal adhesions and 2) cell shape index and its distribution from confocal images of the confluent monolayers of MCF7 cells.

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Keywords: Cell-substrate Adhesion Cell Shape MCF7 Cell Monolayers Quantification Confocal Microscopy ImageJ Focal Adhesion Analysis Background Subtraction Binary Conversion Particle Analysis ImageJ Macro
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