제노이식 모델의 혈액에서 순환 돼지 특이적 DNA의 정량화

Article DOI: 10.3791/61579-v • 07:34 min • September 22nd, 2020

September 22nd, 2020 •

Yangyang Deng*^1,2, Ming Zhou*^1,3, Ying Lu¹, Jiao Chen¹, Zuhui Pu⁴, Dongjing Yu^1,5, Yifan Dai⁶, Yongqiang Zhan⁷, Lisha Mou¹

¹Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, ²Xianning Hospital of Traditional Chinese Medicine, ³Liver-biotechnology (Shenzhen) Co., Ltd., ⁴Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, ⁵Department of Life Sciences, University of Toronto, ⁶Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, ⁷Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital

* These authors contributed equally

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이 프로토콜에서, 돼지 특이적 프라이머는 설계되었고, 플라스미드함유 돼지 특이적 DNA 단편이 시공되었고, 수량에 대한 표준 곡선이 확립되었다. cpsDNA는 종별 프라이머를 사용하여 돼지-마우스 세포 이식 모델 및 돼지-원숭이 동맥 패치 이식 모델에서 qPCR에 의해 정량화되었다.

제노이식 모델의 혈액에서 순환 돼지 특이적 DNA의 정량화

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