Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolation, Proliferation and Differentiation of…
JoVE Journal
Biology

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Biology
Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells
 

Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

Article DOI: 10.3791/61732-v • 07:33 min • May 26th, 2021
May 26th, 2021 •
1,2, 1,2, 1,2
1Department of Physiology, Louisiana State University Health Sciences Center - New Orleans, 2Comprehensive Alcohol-HIV/AIDS Research Center, Louisiana State University Health Sciences Center - New Orleans

Chapitres

résumé
Automatic Translation

In this article, we describe isolation of rhesus macaque derived adipose-derived stem cells (ADSCs) using an enzymatic tissue digestion protocol. Next, we describe ADSC proliferation which includes cell detachment, counting and plating. Lastly, ADSC differentiation is described using specific adipogenic inducing agents. Additionally, we describe staining techniques to confirm differentiation.

Tags

Adipose-derived Stem Cells Rhesus Macaque Isolation Proliferation Differentiation Metabolic Assays Insulin-stimulated Glucose Uptake Lipogenesis Lipolysis Collagenase Digestion RBC Lysis Cell Culture Tissue Processing
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter