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Immunology and Infection

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JoVE Journal Immunology and Infection
A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice
 

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

Article DOI: 10.3791/61867-v • 09:17 min • January 5th, 2021
January 5th, 2021 •
*1,2, *1,2, 3, 4, 5, 5, 2,6, 1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), 2Department of Molecular Medicine, University of Rome “Sapienza”, 3Center for Life Nano Science, Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed
* These authors contributed equally

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résumé
Automatic Translation

Clonal expansion is a key feature of antigen-specific T cell response. However, the cell cycle of antigen-responding T cells has been poorly investigated, partly because of technical limitations. We describe a flow cytometric method to analyze clonally expanding antigen-specific CD8 T cells in spleen and lymph nodes of vaccinated mice.

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Keywords: DNA Ki67 Flow Cytometry Cell Cycle CD8 T Cells Vaccination Immune Dynamics Permeabilization Buffer Hoechst Antigen-Specific
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