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Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis
 

Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis

Article DOI: 10.3791/61892-v • 08:41 min • December 4th, 2020
December 4th, 2020 •
*1, *1, 2, 1
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences, The Pennsylvania State University
* These authors contributed equally

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Here, we describe methods for efficient pupal and adult injections in Nasonia vitripennis as accessible alternatives to embryo microinjection, enabling functional analysis of genes of interest using either RNA-silencing via RNA interference (RNAi) or gene knockout via CRISPR/Cas9 genome editing.

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Keywords: Nasonia Vitripennis Parasitoid Wasp Pupal Injection Adult Injection RNAi CRISPR Gene Editing Functional Genetics Insect Model Non-canonical Model Organism Embryonic Microinjection Genetic Transformation
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