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Biochemistry

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JoVE Journal Biochemistry
Generering og samling af virusspecifikke nukleocapsider af respiratorisk syncytial virus
 
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Generering og samling af virusspecifikke nukleocapsider af respiratorisk syncytial virus

Article DOI: 10.3791/62010-v • 09:08 min • July 27th, 2021
July 27th, 2021 •
1, 1, 1, 1, 1
1Department of Biochemistry, Emory University School of Medicine

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Til dybdegående mekanistisk analyse af respiratorisk syncytial virus (RSV) RNA-syntese rapporterer vi en protokol om anvendelse af chaperonphoprotein (P) til coexpression af RNA-fri nucleoprotein (N0)til efterfølgende in vitro-samling af de virusspecifikke nucleocapsider (NCs).

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Biokemi Problem 173 Generation samling respiratorisk syncytial virus (RSV) virus-specifikke RNA nucleocapsid (NC) chaperone ribonucleoprotein
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