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JoVE Journal Developmental Biology
Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome
 

Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome

Article DOI: 10.3791/62240-v • 09:39 min • December 4th, 2021
December 4th, 2021 •
*1, *1, 2, 1, 1
1Manipal Institute of Regenerative Medicine, Manipal Academy of Higher Education, Manipal, Karnataka, India-576104, 2Department of Medical Genetics, Manipal Hospitals
* These authors contributed equally

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This protocol describes the generation of integration free iPSCs from fetal tissue fibroblasts through delivery of episomal plasmids by nucleofection followed by description of methods used for iPSC characterization and neuronal differentiation.

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Keywords: Turner Syndrome 45XO Fetal Cells Induced Pluripotent Stem Cells IPSCs Aneuploid Reprogramming Chorionic Villi Fibroblasts Episomal Plasmids Cell Culture Differentiation
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