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Developmental Biology

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JoVE Journal Developmental Biology
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
 

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Article DOI: 10.3791/58297-v • 12:06 min • January 11th, 2019
January 11th, 2019 •
1, 1, 1, 2, 1
1Center for Cancer Research, Massachusetts General Hospital, 2Department of Molecular Genetics, Weizmann Institute of Science

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Primordial germ cells (PGCs) are common precursors of both sperm and eggs. Human embryonic PGCs are specified from pluripotent epiblast cells through interactions of cytokines. Here, we describe a 13-day protocol of inducing human cells transcriptomally resembling PGCs at the surface of embryoid bodies from primed-pluripotency induced pluripotent stem cells.

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Keywords: Human Primordial Germ Cell-like Cells Primed-pluripotency Induced Pluripotent Stem Cells Germline Toxicology Genomic Impairment Extracellular Matrix Single-cell Suspension Naive Pluripotency 4i Medium Reproducibility Cell Density Cell Culture Model
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