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JoVE Journal Cancer Research
Modeling Brain Metastasis Via Tail-Vein Injection of Inflammatory Breast Cancer Cells
 

Modeling Brain Metastasis Via Tail-Vein Injection of Inflammatory Breast Cancer Cells

Article DOI: 10.3791/62249-v • 05:02 min • February 4th, 2021
February 4th, 2021 •
*1,2, *1,2, 2,3, 1,2
1Department of Breast Medical Oncology, The University of Texas MD Anderson Cancer Center, 2Morgan Welch Inflammatory Breast Cancer Clinic and Research Program, The University of Texas MD Anderson Cancer Center, 3Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center
* These authors contributed equally

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résumé
Automatic Translation

We describe a xenograft mouse model of breast cancer brain metastasis generated via tail-vein injection of an endogenously HER2-amplified inflammatory breast cancer cell line.

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Modeling Brain Metastasis Tail-vein Injection Inflammatory Breast Cancer Cells Mouse Models Cell Lines Aggressive Form Of Breast Cancer Intracardiac Injection Intracarotid Arterial Injection Advantages Of Tail Vein Method Brain Metastasis Colonization GFP Imaging Disinfect Mouse Remove Fur Cut Cervical Area Of The Neck Excise Skull Harvest Brain Tissue Cassette Cold DPBS Stereo Microscopy UV Light 0.5x Objective Visualization Of Entire Brain Fluorescent Marker
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