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A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
 

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Article DOI: 10.3791/62354-v • 05:34 min • April 15th, 2021
April 15th, 2021 •
1, 2, 1, 1, 1, 3, 1
1Florida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, 2U. S. Fish and Wildlife Service, Pacific Islands Fish and Wildlife Office, 3Department of Entomology and Nematology, University of California Davis

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résumé
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Described here is a DNA extraction protocol using magnetic beads to produce high quality DNA extractions from mosquitoes. These extractions are suitable for a downstream next-generation sequencing approach.

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Magnetic Bead-based DNA Extraction Protocol Next-generation Sequencing High Throughput Sequencing Budget-friendly Resource Limited Labs Diagnostic Research High Quality DNA Extraction PCR Grade Water Proteinase K Buffer Enzyme Mix Microcentrifuge Tube Homogenize Tissue Centrifuge Tissue Lysate Magnetic Bead Master Mix
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