Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Immunology and Infection

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
 

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Article DOI: 10.3791/62368-v 07:42 min April 16th, 2021
April 16th, 2021

Chapitres

résumé

A protocol is presented to extract the total lipid content of the cell wall of a wide range of mycobacteria. Moreover, extraction and analytical protocols of the different types of mycolic acids are shown. A thin-layer chromatographic protocol to monitor these mycobacterial compounds is also provided.

Tags

Keywords: Mycobacteria Lipids Thin Layer Chromatography Extraction Chloroform Methanol Mycolic Acid N-hexane TLC
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter