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Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
 

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Article DOI: 10.3791/62510-v 08:57 min July 17th, 2021
July 17th, 2021

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résumé

The baculovirus expression vector system (BEVS) is a robust platform for expression screening and production of protein arginine methyltransferases (PRMTs) to be used for biochemical, biophysical, and structural studies. Milligram quantities of material can be produced for the majority of PRMTs and other proteins of interest requiring a eukaryotic expression platform.

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Keywords: Recombinant PRMT Proteins Baculovirus Expression Vector System Protein Expression SF9 Cells Transfection Serum-free Insect Media Protein Purification Biochemical Studies Biophysical Studies Structural Studies
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