JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
実験性脂肪化のモデル
:
脂質過負荷状態培地における肝細胞細胞培養
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
A Model of Experimental Steatosis
In Vitro
: Hepatocyte Cell Culture in Lipid Overload-Conditioned Medium
Please note that all translations are automatically generated.
Click here for the English version.
実験性脂肪化のモデル
:
脂質過負荷状態培地における肝細胞細胞培養
DOI:
10.3791/62543-v
•
08:35 min
•
May 18, 2021
•
Adriana Campos-Espinosa
,
Carolina Guzmán
1
Laboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina-UNAM
,
Hospital General de México “Dr. Eduardo Liceaga”
Chapters
00:05
Introduction
00:55
Standard and Conditioned Medium Preparation
03:11
Steatogenic Culture
03:48
Viability and Mortality Assessment
04:44
Lipid Staining with Oil-Red O
05:46
Morphometric Assessment of Lipid Contents
06:29
Results: Hepatocyte Culture in Steatogenic Conditions
07:44
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
このプロトコルは、肝細胞の脂質への過剰暴露によって産生される脂肪、生化学的、細胞変化をイン
ビトロで
ステアトーシスおよび分子、生化学的、細胞変化を研究するためのツールとなるものである。
Tags
Liver Steatosis
In Vitro
Hepatocyte Cell Culture
Lipid Overload
Palmitate
Oleate
Steatogenic Medium
Mild Steatosis
Severe Steatosis
RPMI 1640
Fatty Acids
Lipid-free BSA
Reproducibility
Diagnostic Markers
Therapeutic Targets
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/62543?article_type=v
口腔扁平上皮癌の同所性マウスモデルにおける腫瘍細胞の浸潤の多光子イメージング
check_url/62543?article_type=v
における幹細胞移植
in vitroで
模擬虚血/再灌流モデル
check_url/62543?article_type=v
胎児性アルコール暴露のゼブラフィッシュモデルの催奇形性変化を評価する
check_url/62543?article_type=v
in vitroで
中皮クリアランスアッセイ
で
そのモデルの卵巣がんの転移の初期段階を
check_url/62543?article_type=v
脳Microslicesの興奮毒性刺激<em>インビトロ</em>脳卒中のモデル
check_url/62543?article_type=v
脊髄損傷の実験的挫傷モデルにおける神経幹細胞移植
check_url/62543?article_type=v
の最適化分析<em>インビボ</em>と<em>インビトロ</em>脂肪肝
check_url/62543?article_type=v
体外
配信生細胞イメージング化学療法を探求する脂質ナノ粒子によってを使用してください。
check_url/62543?article_type=v
抵抗血管の細胞培養モデル
check_url/62543?article_type=v
郭清と尿の添付ファイルの
In Vitro
解析マウス尿膜の培養
Read Article