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実験性脂肪化のモデル
:
脂質過負荷状態培地における肝細胞細胞培養
JoVE Journal
Medicine
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JoVE Journal
Medicine
A Model of Experimental Steatosis
In Vitro
: Hepatocyte Cell Culture in Lipid Overload-Conditioned Medium
Please note that all translations are automatically generated.
Click here for the English version.
実験性脂肪化のモデル
:
脂質過負荷状態培地における肝細胞細胞培養
DOI:
10.3791/62543-v
•
08:35 min
•
May 18, 2021
•
Adriana Campos-Espinosa
,
Carolina Guzmán
1
Laboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina-UNAM
,
Hospital General de México “Dr. Eduardo Liceaga”
Chapters
00:05
Introduction
00:55
Standard and Conditioned Medium Preparation
03:11
Steatogenic Culture
03:48
Viability and Mortality Assessment
04:44
Lipid Staining with Oil-Red O
05:46
Morphometric Assessment of Lipid Contents
06:29
Results: Hepatocyte Culture in Steatogenic Conditions
07:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、肝細胞の脂質への過剰暴露によって産生される脂肪、生化学的、細胞変化をイン
ビトロで
ステアトーシスおよび分子、生化学的、細胞変化を研究するためのツールとなるものである。
Tags
Keywords: Liver Steatosis
In Vitro
Hepatocyte Cell Culture
Lipid Overload
Palmitate
Oleate
Steatogenic Medium
Mild Steatosis
Severe Steatosis
RPMI 1640
Fatty Acids
Lipid-free BSA
Reproducibility
Diagnostic Markers
Therapeutic Targets
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