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体外实验性硬化模型：脂质超载介质中的肝细胞培养

JoVE Journal
Medicine

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JoVE Journal Medicine

A Model of Experimental Steatosis In Vitro: Hepatocyte Cell Culture in Lipid Overload-Conditioned Medium

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体外实验性硬化模型：脂质超载介质中的肝细胞培养

DOI:

10.3791/62543-v

•

08:35 min

•

May 18, 2021

•

Adriana Campos-Espinosa, Carolina Guzmán

¹Laboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina-UNAM,Hospital General de México “Dr. Eduardo Liceaga”

Chapters

00:05Introduction
00:55Standard and Conditioned Medium Preparation
03:11Steatogenic Culture
03:48Viability and Mortality Assessment
04:44Lipid Staining with Oil-Red O
05:46Morphometric Assessment of Lipid Contents
06:29Results: Hepatocyte Culture in Steatogenic Conditions
07:44Conclusion

Summary

Automatic Translation

该协议旨在成为研究硬化症和分子，生化，细胞变化产生的肝细胞过度暴露 在体外脂质的工具。

Tags

Keywords: Liver Steatosis In Vitro Hepatocyte Cell Culture Lipid Overload Palmitate Oleate Steatogenic Medium Mild Steatosis Severe Steatosis RPMI 1640 Fatty Acids Lipid-free BSA Reproducibility Diagnostic Markers Therapeutic Targets

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