Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Direct Detection of Isolevuglandins in Tissues…
JoVE Journal
Biology

Ce contenu est en libre accès.

JoVE Journal Biology
Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
 

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

Article DOI: 10.3791/62603-v • 06:33 min • July 5th, 2021
July 5th, 2021 •
*1, *2, 3, 4,6, 4, 5, 3, 4,6
1Vanderbilt Eye Institute, Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University, 3Department of Biochemistry, Vanderbilt University, 4Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center, 5Division of Hematology and Oncology, Indiana University School of Medicine, 6Department of Molecular Physiology and Biophysics, Vanderbilt University
* These authors contributed equally

Chapitres

résumé
Automatic Translation

This article provides a detailed methodology for the measurement of isolevuglandins in tissues by immunofluorescence using alkaline phosphatase-conjugated ScFv D11 antibody. Hypertension models in both mice and humans are used to explain the step-by-step procedures and fundamental principles associated with isolevuglandin measurement in tissue samples.

Tags

D11 ScFv-Alkaline Phosphatase Fusion Protein Immunofluorescence Detection Protocol Cardiovascular Disease Secondary Antibody Xylene Deparaffinization Tissue Rehydration Ethanol Washes Tris-buffered Saline Tween 20 Sodium Citrate Buffer Antigen Retrieval Incubation Blocking Slides BSA
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter