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Imagem intravital longitudinal através de janelas de silicone claras
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1Cold Spring Harbor Laboratory, 2Cold Spring Harbor Laboratory School of Biological Sciences, 3Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 4Medical Scientist Training Program, School of Medicine,Stony Brook University, 5Graduate Program in Pharmacology,Stony Brook University, 6Graduate Program in Genetics,Stony Brook University, 7Graduate Program in Biomedical Engineering,Stony Brook University, 8Department of Biomedical Engineering,Stony Brook University, 9Department of Pathology,University of Michigan
Chapitres
- 00:04Introduction
- 00:50Casting the Silicone Window and Preparing the Mouse for Insertion of the Silicone Window
- 01:44Inserting a Lateral Window for Imaging in the Liver and the Pancreas
- 03:51Inserting the Window for Imaging in the Mammary Gland
- 04:56Imaging Through the Window
- 06:04Results: Investigation of the Adaptability of the PDMS Window to Diverse Tissue Sites
- 07:05Conclusion
Uma abordagem é aqui apresentada para imagens intravitais de longo prazo usando janelas de silicone opticamente claras que podem ser coladas diretamente ao tecido/órgão de interesse e à pele. Essas janelas são mais baratas e mais versáteis do que outras atualmente utilizadas no campo, e a inserção cirúrgica causa inflamação e angústia limitadas aos animais.
