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Immunology and Infection

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JoVE Journal Immunology and Infection
An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis
 

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

Article DOI: 10.3791/62838-v • 09:34 min • August 16th, 2021
August 16th, 2021 •
1, 1,2, 2, 2,3,4, 1, 1
1Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James's Hospital, Trinity College Dublin, The University of Dublin, 2School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), 3SFI Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, 4SFI Centre for Research in Medical Devices (CURAM), RCSI, Dublin and National University of Ireland

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Rapid and efficient quantification of intracellular M. tuberculosis growth is crucial for pursuing improved therapies against tuberculosis (TB). This protocol describes a broth-based colorimetric detection assay using an automated liquid culture system to quantify Mtb growth in macrophages treated with candidate host-directed therapies.

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Keywords: Automated Culture System Host-directed Therapies Tuberculosis Mycobacterium Tuberculosis Intracellular Growth FDA-approved Drugs Repurposing Mycobacterial Culture EO Carrier T-cell Culture Attenuated Mycobacterial Culture H37Ra Centrifugation RPMI Resuspension Mycobacterial Suspension Glass Chamber Slides Phagocytosis PBS PFA
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