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Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
 

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Article DOI: 10.3791/62854-v • 10:07 min • October 8th, 2021
October 8th, 2021 •
1,2, 2, 3, 2
1UES Inc., 2711th Human Performance Wing, Airman Systems Directorate, Air Force Research Laboratory, Wright-Patterson AFB, 3Materials and Manufacturing Directorate, Wright-Patterson AFB

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The protocol describes the formation of robust and biocompatible DNA-laden microcapsules as multiplexed in vitro biosensors capable of tracking several ligands.

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Keywords: Multifunctional Silk-based Microcapsules DNA Plasmids RNA Aptamers Riboswitches Biocompatible Microcapsules DNA Immobilization Biosensors Biological Processes Signaling Molecules Gene Activation Polyethyleneimine Silica Microparticles DNA Plasmids Theophylline Riboswitch Broccoli Aptamer Silk Fibroin
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