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JoVE Journal Developmental Biology
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
 

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Article DOI: 10.3791/62865-v • 09:56 min • August 31st, 2021
August 31st, 2021 •
1, 2,3, 1, 2,4, 1
1Neurophotonics and Mechanical Systems Biology, Institut de Ciències Fotòniques, ICFO, 2Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 3Institut de Ciències Fotòniques, ICFO, 4Universitat Pompeu Fabra (UPF)

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Here, we present a protocol to investigate the intracellular mechanical properties of isolated embryonic zebrafish cells in three-dimensional confinement with direct force measurement by an optical trap.

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Keywords: Optical Tweezers Subcellular Mechanics Cell Nucleus Cell Polarization Cell Differentiation Cell Migration Viscoelastic Medium Cell Preparation Hoechst Dye DMEM Optical Trapping OT Chamber
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