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JoVE Journal Cancer Research
Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
 

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates

Article DOI: 10.3791/62875-v • 11:32 min • August 6th, 2021
August 6th, 2021 •
1,2,3, 1, 2,4, 1,3
1Integrated Physiology and Molecular Medicine Laboratory, Pennington Biomedical Research Center, 2Clinical Oncology and Metabolism, Pennington Biomedical Research Center, 3Department of Nutrition, Case Western Reserve University, 4Department of Clinical Nutrition, University of Texas Southwestern Medical Center

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résumé
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We developed a practical protocol and analytical approach to evaluate mitochondrial oxidative phosphorylation and electron transfer capacity in fresh tumor homogenates. This protocol can be easily adapted to survey various mitochondrial functions that contribute to cancer initiation, progression, and treatment response.

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Keywords: Mitochondrial Function Tumor Homogenates Tissue Homogenization Mitochondrial Respiration Biopsy Preservation Tissue Sections Tissue Homogenate Quantitative Assessment Clinical Translational Oncology Diagnostic Interpretation Cancer Progression
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