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Immunology and Infection

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JoVE Journal Immunology and Infection
Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
 

Generating Transgenics and Knockouts in Strongyloides Species by Microinjection

Article DOI: 10.3791/63023-v • 09:42 min • October 7th, 2021
October 7th, 2021 •
1, 1,2
1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

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résumé
Automatic Translation

The functional genomic toolkit for the parasitic nematodes Strongyloides stercoralis and Strongyloides ratti includes transgenesis, CRISPR/Cas9-mediated mutagenesis, and RNAi. This protocol will demonstrate how to use intragonadal microinjection to introduce transgenes and CRISPR components into S. stercoralis and S. ratti.

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Keywords: Microinjection Transgenics Knockouts Strongyloides Parasites Worms Baermann Fecal-charcoal Nematode Growth Medium E. Coli HB101
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